This page is general health information, not medical advice. It cannot tell you whether you have an infection. A clinician can, and the testing options on this site are a good place to start.

Orlando is spread out, and the right clinic is often the one that fits your commute, your schedule and your budget. This guide walks you through choosing a testing site by area, then preparing for the visit and understanding what comes after.

It is organized as steps. Follow them in order, or jump to the area where you live or work. For the full list of sites in one place, see the local testing list.

What you will need before you go

A photo ID. The Florida Department of Health in Orange County says its STD clinic requires a picture ID.
A way to pay, or a question about cost. Some sites are free and some charge fees. Details are in each area below.
Insurance or Medicaid or Medicare cards, if you have them. Several clinics accept them. None is required at every site.
A phone you can use for results. The county clinic says results can be given by coded text with signed consent, or in person.
A short list of notes. Write down the date of your last test, any symptoms you have noticed, and questions for the clinician.
Flexible time. Walk-in clinics can have lines.

Step 1: Decide what you want to be tested for

Different sites offer different tests. That matters more in Orlando than you might expect, because the menu changes from one address to the next.

For example, Miracle of Love's Test & Treat page says the organization does not perform swabs for gonorrhea or chlamydia and points swab testing to the Department of Health. A site that offers HIV and syphilis testing may therefore not cover everything you have in mind.

If you are not sure what to ask for, a clinician can talk through your situation and suggest which tests make sense. Tell them about the kinds of sex you have and the body sites involved. You do not need to explain more than you want to.

Step 2: Pick your area

Central Boulevard and downtown

The Florida Department of Health in Orange County STD Clinic is at 832 West Central Blvd, Orlando, FL 32805. The county's STD page lists low- or no-cost testing for chlamydia, gonorrhea, syphilis and HIV. A CDC GetTested listing for the same site also lists rapid HIV and hepatitis tests.

Here is what the county page says about how it works:

It is walk-in, first come, first served.
Hours are listed as Monday to Friday, 7:30 a.m. to 4 p.m., closed noon to 1 p.m. for lunch.
Fees are listed as starting at $20, and a sliding scale is available after an eligibility screening.
Payment can be cash, card, Medicaid or Medicare.
Results take about one week.

Other county pages list different hours, so call 407-858-1400 before you go.

Also in the 32805 area, Orange Blossom Family Health is at 232 N Orange Blossom Trail (407-428-5751). Its CDC GetTested listing shows rapid HIV, hepatitis and STI tests, a sliding scale, and that an appointment is required. Its own website lists HIV testing as free but does not mention STD testing, so confirm by phone. Hours are not published in the sources reviewed.

Colonial Drive and the Seminole Avenue area

Miracle of Love, an HIV/AIDS nonprofit, has two screening sites in 32804:

Executive Office, 1301 W Colonial Dr, Ste 201, (407) 843-1760. Screenings are listed Monday to Thursday, 9:00 to 11:30 a.m. and 1:00 to 5:00 p.m., and Friday, 9:00 a.m. to noon. CDC GetTested lists free HIV and STI tests and says an appointment is required.
Stafford House, 711 Seminole Ave, (407) 532-0070. Screenings are listed Monday to Thursday, 12:00 to 3:30 p.m. and 5:00 to 7:30 p.m., and Friday, 2:30 to 5:30 p.m. The page does not state a price or walk-in policy, and another page gives slightly different hours, so call first.

Nearby, AIDS Healthcare Foundation opened a center at 707 E. Colonial Drive. Its September 2025 announcement says it offers free STD/STI prevention and treatment services and access to PrEP, Monday to Friday, 8:00 a.m. to 5:00 p.m. No phone number or walk-in policy was published in that release, so check with AHF before you go.

Mills Avenue (Mills 50)

The Center Orlando, an LGBTQ+ community center, runs a testing center at 942 N Mills Ave, Orlando, FL 32803. Its testing page says all sexual health testing is walk-in only. It publishes these prices:

HIV: $0
Hepatitis C: $0
Syphilis: $15
Chlamydia and gonorrhea: $50
Syphilis, chlamydia and gonorrhea bundle: $55

Hours on its homepage are Monday and Wednesday and Friday, 9 a.m. to 8 p.m.; Tuesday and Thursday, 9 a.m. to 5 p.m.; Sunday, 10 a.m. to 2 p.m.; and closed Saturday. HIV and hepatitis C testing is done by The Center, and STI testing by NPS Healthcare. A phone number was not listed in the sources reviewed.

Raleigh Street, west of downtown

The Florida Department of Health in Orange County also runs the Lila Mitchell Health Center at 5151 Raleigh St, Ste B, Orlando, FL 32811, phone (407) 723-5004. The CDC GetTested listing shows HIV, chlamydia, gonorrhea and syphilis tests, a sliding scale, and that insurance, Medicaid and Medicare are accepted. It says an appointment is required. Hours were not found, so call to schedule.

East Orlando and University Boulevard

The CDC's NPIN listing shows Planned Parenthood East Orlando Health Center at 11500 University Blvd, Ste B, Orlando, FL 32817, phone 407-246-1788. It lists chlamydia, gonorrhea, herpes, rapid HIV and syphilis tests, and says an appointment is required. Hours and prices differ between directories, so confirm both with the health center directly.

Winter Park, just north of the city

Hope & Help Center of Central Florida is at 4122 Metric Dr, #800, Winter Park, FL 32792, (407) 645-2577. It sits in Orange County but outside Orlando city limits, and it serves the Orlando area. Its testing page lists HIV, chlamydia, syphilis, gonorrhea, herpes, hepatitis C and HPV, and says testing is free with no appointment necessary. Testing hours are Monday to Thursday, 8:00 a.m. to 4:00 p.m. The page has some copy errors in its test descriptions, so confirm the specific tests by phone.

Step 3: Call ahead and ask four questions

Which tests do you offer, including swabs and blood draws?
What will it cost me, and is there a sliding scale?
Do I need an appointment, or can I walk in?
How and when will I get my results?

Published hours and policies can lag behind real life. A two-minute call can save you a trip.

Step 4: Know your privacy rights

Under Florida Statutes section 384.30, a parent's or guardian's consent is not a prerequisite for a minor's examination or treatment for a sexually transmissible disease. The statute states no minimum age. It also says the fact of a minor's consultation, examination and treatment is confidential and may not be divulged, such as by sending a bill to a parent or guardian, except as provided in section 384.29.

Florida law also requires licensed practitioners and laboratories to report certain infections to the Department of Health. According to Florida Administrative Code Rule 64D-3.029, these include chlamydia, gonorrhea, syphilis and HIV. If you are concerned about what is shared, ask the clinic to explain.

Step 5: Go to your visit and follow up

Arrive early at walk-in sites. The county clinic notes that numbers are assigned quickly after opening. Bring your ID and anything else from the materials list above.

A clinician may ask about your sexual history and symptoms. Those questions help them choose the right tests. You can ask them to explain anything.

Then follow through on results. If a result comes back positive, a clinician can explain treatment options. Chlamydia, gonorrhea and syphilis are curable with treatment prescribed by a clinician, according to the CDC. HIV and herpes are not curable but are treatable and manageable. Florida's expedited partner therapy rule, section 384.27, lets a clinician provide treatment for a partner in certain situations, per the CDC.

Tips and common mistakes

Assuming every site tests for everything. The menus differ, as the Miracle of Love swab note shows.
Showing up without ID at the county clinic. The county says a picture ID is required.
Trusting a single published hours listing. Several Orlando sites have listings that disagree, so call first.
Arriving at lunch. The county clinic closes from noon to 1 p.m.
Skipping the cost question. Prices range from free to the published fees above. Ask what applies to you.
Forgetting to ask when to test again. A clinician can tell you whether a repeat test makes sense for your situation, and the CDC provides guidance on timing for each infection.
Waiting out of embarrassment. Clinicians see these questions every day. Testing is a routine part of health care.

What to expect afterward

Results may come back in about a week at the county clinic, according to its page. Other sites will tell you their own timing. Many people find that having a plan for how results will reach them makes the wait easier.

A negative result may suggest that no infection was detected at the time of the test. It does not rule out recent exposure, and a clinician can explain what it does and does not mean for you. A positive result can usually be followed by a conversation about treatment and next steps.

To see how Orange County compares with the rest of Florida, read our look at STD rates in Orlando. For context, FLHealthCHARTS reports a 2025 chlamydia rate of 516.7 per 100,000 in Orange County, compared with 410.8 per 100,000 statewide. Rates describe a community. They do not describe any one person.

STD Testing in Orlando: A Neighborhood-by-Neighborhood Guide