This page is general health information, not medical advice. It cannot tell you whether you have an infection. A clinician can, and the testing options on this site are a good place to start.

Orlando STD testing is spread across several parts of Orange County. Some clinics are downtown, one is in Winter Park, and Kissimmee sits just south in Osceola County. Each option works a little differently.

This guide shows you how to pick a site by area, what to prepare, and what to expect on the day. It is a planning tool, not a list of every clinic. For the full local list, see the Orlando testing list.

What you need before you go

A photo ID. The Florida Department of Health in Orange County says picture ID is required at its STD clinic.
A way to pay, or a question about cost. The county clinic accepts cash, card, Medicaid and Medicare. Insurance details can vary by site, so ask when you call.
A phone you can use for results. The county clinic can give results by coded text if you sign consent. Otherwise results are given in person.
A short list of what you want tested. Not every site offers every test. A few notes on that are below.
A rough idea of your travel time. Some sites take walk-ins only. Others need an appointment.

Step 1: Decide which area works best for you

Start with where you will be during the day. Clinic hours are mostly weekday hours, so a site near work or home saves time.

Downtown and central Orlando

This area has the most options, and they differ in cost and style.

Florida Department of Health in Orange County STD Clinic, 832 West Central Blvd, Orlando, FL 32805. The county STD page says it is walk-in, first come first served. You can call 407-858-1400 to schedule. The page lists Monday to Friday, 7:30 a.m. to 4 p.m., closed 12 to 1 p.m. for lunch.
Miracle of Love, 1301 W Colonial Dr, Ste 201. Its locations page lists HIV and STI screenings Monday to Thursday and Friday morning. The phone is (407) 843-1760.
Miracle of Love, Stafford House, 711 Seminole Ave. Screenings are listed in the afternoon and evening, Monday to Thursday, and Friday late afternoon. The phone is (407) 532-0070. The organization's Test & Treat page lists slightly different hours, so confirm by phone.
Orange Blossom Family Health, 232 N Orange Blossom Trail. The CDC GetTested listing shows an appointment is required. The phone is 407-428-5751.
The Center Orlando, 942 N Mills Ave. Its testing page says all sexual health testing is walk-in only. The phone number is not published in the sources I reviewed, so check its website.
AHF Healthcare Center, 707 E. Colonial Drive. An AHF announcement from September 2025 describes free STD/STI prevention and treatment services here. It does not give a phone number or say whether walk-ins are accepted.

Winter Park

Hope & Help Center of Central Florida is at 4122 Metric Dr, #800, Winter Park, FL 32792. It serves the Orlando area from just outside city limits. Its testing page lists testing Monday to Thursday, 8 a.m. to 4 p.m. It also says no appointment is necessary and that testing is free. The phone is (407) 645-2577.

The page lists a wide range of tests. It also has some copy errors in the test descriptions, so call to confirm the exact tests before you go.

Kissimmee

Kissimmee is in Osceola County, so the Orange County health department does not serve it. This guide does not list a Kissimmee clinic, because I could not confirm site details such as hours, prices or walk-in rules. Rather than guess, I suggest two options:

Contact the Florida Department of Health in Osceola County through the state health department website and ask where STD testing is offered.
Use the CDC's GetTested locator to look up sites by ZIP code, then call to confirm.

From Kissimmee, the downtown Orlando sites above are one possible fallback. Check travel time and hours first.

Step 2: Match the site to the tests you want

Services differ from site to site. Here are the points that matter most.

The county clinic page lists low- or no-cost testing for chlamydia, gonorrhea, syphilis and HIV. The CDC GetTested listing for the same site adds rapid HIV and hepatitis B and C tests.
Miracle of Love's Test & Treat page says it does not perform swabs for gonorrhea or chlamydia. It points swab testing to the Department of Health. If you want those tests, plan accordingly.
The Center Orlando publishes prices: HIV $0, hepatitis C $0, syphilis $15, chlamydia and gonorrhea $50, and a three-test bundle of syphilis, chlamydia and gonorrhea for $55. Its page says STI testing is done by NPS Healthcare.
The Lila Mitchell Health Center, 5151 Raleigh St, Ste B, is listed by CDC GetTested with HIV, chlamydia, gonorrhea and syphilis tests. An appointment is required. The phone is (407) 723-5004.
Planned Parenthood East Orlando, 11500 University Blvd, Ste B, is listed in a CDC directory with an appointment required. The phone is 407-246-1788. I could not confirm current hours on its own site, so call first.

Step 3: Call or check the site before you travel

Hours change, and published sources do not always agree. The county STD page shows one schedule, while other county pages and listings showed different ones. A two-minute call can prevent a wasted trip.

Ask these questions:

Are you open today, and what time should I arrive?
Do I need an appointment, or can I walk in?
Do you offer the specific tests I want?
What will it cost, and do you take my insurance or offer a sliding scale?
How and when will I get results?

Step 4: Plan for the day

At the county clinic, the page says numbers are assigned quickly after opening. For a walk-in, arriving early is the safer plan. Allow extra time, since first come first served can mean a wait.

Bring your ID and your payment method. Wear something comfortable. A clinician or staff member will explain which samples they need, which can include blood, urine or a swab, depending on the tests.

Step 5: Know what the cost may look like

Costs vary a lot across these sites.

The county page says clinic fees range from $20 and up, with a sliding scale after an eligibility screening.
Hope & Help calls its testing free. It accepts most major insurances, and income-based self-pay options can be arranged.
Miracle of Love is listed by CDC GetTested as offering free HIV and STI tests.
Orange Blossom Family Health says it uses a sliding fee scale based on household income. It also says no patient will be turned away because of inability to pay.

If a price is not published, the page simply does not list it. Ask the clinic directly.

Step 6: Plan how you will get your results

The county STD page says results take about one week. They are given in person or by coded text with signed consent. Ask each clinic what its process is, and which phone number it will use.

If a test result comes back positive, a clinician can explain next steps. Bacterial STIs and trichomoniasis can be cured with treatment a clinician prescribes. HIV, herpes and HPV cannot be cured, but they are treatable or manageable. Several clinics above list treatment, so ask whether follow-up care happens at the same site.

Tips for a smoother visit

Go early for walk-in sites. First come first served favors the first people through the door.
Check lunch closures. The county clinic closes from 12 to 1 p.m.
Ask about PrEP and PEP while you are there. Miracle of Love lists low-cost PEP and PrEP, and says PEP must start within 72 hours of exposure. Hope & Help lists access to PrEP and nPEP. The Florida Department of Health Area 7 program also links a PrEP and PEP locator.
Consider a free at-home HIV kit. The county Area 7 HIV/AIDS Program page offers a free at-home kit order. Eligibility is not stated on the page, so confirm it with the program.
Know your privacy rights if you are a minor. Florida Statute 384.30, on the Florida Legislature's Online Sunshine site, says a parent's consent is not required for STD examination or treatment. It also says the fact of a minor's care is confidential and may not be shared, for example by sending a bill to a parent.

Common mistakes to avoid

Trusting one listing for hours. Sources disagree, so call.
Assuming a site offers every test. Swab testing in particular varies.
Forgetting ID. The county clinic requires it.
Skipping the cost question. Prices run from free to a few dozen dollars per test.
Assuming Winter Park is inside Orlando. It is a short trip from downtown, but a separate city.
Waiting for a perfect time. A clinician can help you decide what to test for and when.

What to expect afterward

Many people receive results within about a week at the county clinic. Other sites may differ. A negative result generally means the test did not find the infection it checked for. A clinician can tell you whether repeat testing makes sense for your situation. A positive result is something a clinician can explain and treat or help manage.

For context on why testing matters locally, the Florida Department of Health's FLHealthCHARTS reported a 2025 chlamydia rate of 516.7 per 100,000 people in Orange County, compared with 410.8 for Florida. You can read more in our breakdown of the latest Orlando STD data.

Quick checklist

Pick an area: downtown, Winter Park or a Kissimmee-area option.
Match the site to the tests you want.
Call to confirm hours, appointments, cost and tests.
Bring photo ID and a payment method.
Ask how and when you will get results.

STD Testing Across Orlando: Downtown, Winter Park and Kissimmee Options